A selectable marker is used in the section of recombinants on the basis of their ability to produce colour in presence of chromogenic substrate.

0 votes
29 views
asked Dec 6, 2017 in Biology by sforrest072 (157,439 points) 60 409 933
A selectable marker is used in the section of recombinants on the basis of their ability to produce colour in presence of chromogenic substrate.
(a) Mention the name of mechanism involved.
(b) Which enzyme is involved in production of colour ?
(c) How is it advantageous over using antibiotic resistant gene as a selectable market ?

1 Answer

+1 vote
answered Dec 6, 2017 by mdsamim (213,225 points) 5 10 15
selected Feb 23, 2018 by sarthaks
 
Best answer

a) Insertional inactivation
(b)β -Galactosidase.
(c) Selection of recombinants due to inactivation of antibiotics requires
simultaneous plating on two plates having different antibiotics.

Welcome to Sarthaks eConnect: A unique platform where students can interact with teachers/experts/students to get solutions to their queries. Students (upto class 10+2) preparing for All Government Exams, CBSE Board Exam, ICSE Board Exam, State Board Exam, JEE (Mains+Advance) and NEET can ask questions from any subject and get quick answers by subject teachers/ experts/mentors/students.

One Thought Forever

“There is a close connection between getting up in the world and getting up in the morning.“
– Anon
~~~*****~~~

...