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What is the significance of adding proteases at the time of isolation of genetic material (DNA).

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asked Dec 6, 2017 by mdsamim (213,225 points) ● 5 ● 10 ● 15
edited Mar 6, 2018 by faiz
What is the significance of adding proteases at the time of isolation of genetic material (DNA).

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answered Dec 6, 2017 by mdsamim (213,225 points) ● 5 ● 10 ● 15
selected Feb 24, 2018 by sarthaks
 
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Role of proteases is to degrade the proteins present inside a cell (from which DNA is being isolated). If the proteins are not removed from DNA preparation then they could interfere with any downstream treatment of DNA (such action of restriction endonuclease, DNA ligase etc).

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