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While doing a PCR, ‘denaturation’ step is missed.

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asked Dec 6, 2017 in Biology by mdsamim (213,225 points) ● 5 ● 10 ● 15
edited Mar 6, 2018 by faiz
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

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+1 vote
answered Dec 6, 2017 by mdsamim (213,225 points) ● 5 ● 10 ● 15
edited Mar 6, 2018 by faiz
 
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If denaturation of double-stranded DNA does not take place, then primers will not be able to anneal to the template, no extension will take place, hence no amplification will occur.

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