Question

You have identified a useful gene in a bacteria. Make a flowchart of the steps that you would follow to transfer this gene to a plant.

Answer 1

After identifying a useful gene in bacteria, following steps should be undertaken

(1) Isolation of useful gene using Restriction Endonucleases
                     ↓
(2) Transferring the gene to a suitable vector to create a recombinant DNA molecule
                     ↓
(3) Transfer of these recombinant DNA molecules to the target cells
                    ↓
(4) Screening of cells for transformation
                    ↓
(5) Selection of transformed cells
                    ↓
(6) Regeneration of plants from the transformed cells to get transgenic plants.