You are repeating the Hershey-Chase experiment and are provided with two isotopes

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asked Dec 8, 2017 in Biology by sforrest072 (157,439 points) 60 409 936

You are repeating the Hershey-Chase experiment and are provided with two isotopes: 32P and 15N (in place of35S in the original experiment). How do you expect your results to be different?

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answered Dec 8, 2017 by mdsamim (213,225 points) 5 10 15
selected Feb 26, 2018 by sarthaks
 
Best answer

Use of 15N will be inappropriate because method of detection of 35p and 15N is different (32p being a radioactive isotope while 15N is not radioactive but is the heavier isotope of nitrogen). Even if 15N was radioactive then its presence would have been detected both inside the cell (15N incorporated as nitrogenous base in DNA) as well as in the supernatant because 15Nwould also get incorporated in amino group of amino acids in proteins). Hence the use of 15N would not give any conclusive results.

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