Study the figure of vector pBR322 given below in which foreign DNA is ligated at the Bam HI site of tetracyline resistance gene.

Question

Study the figure of vector pBR322 given below in which foreign DNA is ligated at the Bam HI site of tetracycline resistance gene.

(a) Mention the function of rop.
(b) What will be the selectable marker for this recombinant plasmid and why ?
(c) Explain transformation

Answer 1

(a) ‘Rop’ codes for the proteins involved in the replication of plasmid
(b) Selectable marker : Ampicillin resistance gene. It will help distinguishing transformants from non-transformants after plating them on ampicillin-containing medium.
(c) Transformation: It is the phenomenon by which the DNA isolated from one type of cell and introduced into another type, is able to bring about some of the properties of former to the later.