Question

Observe the given sequence of nitrogenous bases on a DNA fragment and answer the following questions.
5'- CAGAATTCTTA-3'
3'- GTCTTAAGAAT-5'

(a) Name of restriction enzyme which can recognize this DNA sequence.
(b) Write the sequence after digestion.
(c) Why are the ends generated after digestion called sticky ends?

Answer 1

(a) EcoRI

(c) These are named sticky ends, because they form hydrogen bonds with their complementary cut parts.