(i) Chemical treatment: treated with divalent cation such as Calcium) and
exposure to cold and high temp. (42° C) alternatively (Bacterial cell)
(ii) Biolistics or gene gun. (Plant cell). In this method gold and tunguston
particles, coaled with DNA are bombarded with high velocity.
(iii) Micro-injection, (animal cell). In this method r DNA is directly injected
into the nucleus of an animal cell.