Question

During in vitro synthesis of DNA, a researcher used 2’, 3’ – dideoxy cytidine triphosphate as raw nucleotide in place of 2’-deoxy cytidine triphosphate other conditions remaining as standard. Will further polymerisation of DNA continue upto the end or not? Explain.

Answer 1

Further polymerisation would not occur, as the 3’ OH on sugar is not there to add a new nucleotide for forming ester bond.