Question

During in vitro synthesis of DNA, a researcher used 2’, 3’–dideoxy cytidine triphosphate as raw nucleotide in place of2’-deoxy cytidine triphosphate other conditionsremainingas standard. Will furtherpolymerisation of DNA continue upto the end ornot? Explain.

Answer 1

Further polymerisation would not occur, as the 3’OH on sugar is not there to add a newnucleotide for forming ester bond.