Question

Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

Answer 1

i. Restriction endonucleases: for cutting the desired DNA at desired places

 ii. Gel electrophoresis: for separating the desired DNA fragments

 iii. Ligase enzyme: for creating recombinant DNA molecule.

 iv. DNA delivery system: like electroporation, microinjection, gene gun method.

 v. Competant host (usually bacteria / yeast): to take up recombinant DNA