Question

Name and explain the technique that heips in the separation of DNA fragments for DNA recombinant technology experiments. How can these separated DNA fragments be visualised?

Answer 1

Gel electrophoresis , Since DNA fragments are negatively charged , they move towards anode (under an electric field ) through a medium / matrix / agarose gel , The fragments separate (resolve) according to their size through sieving effect provided by agarose gel , The separated DNA fragments can be visualised after staining the DNA with ethidium bromide , followed by exposure to UV radiation