Name and describe the technique that helps in separation and isolation of DNA fragments for biotechnology experiment.

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asked Jan 31, 2018 in Biology by Rohit Singh (61,782 points) 35 133 356

Name and describe the technique that helps in separation and isolation of DNA fragments for biotechnology experiment.

1 Answer

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answered Jan 31, 2018 by sameer (82,980 points) 5 11 37
selected Feb 4, 2018 by Vikash Kumar
 
Best answer

Gel - electrophoresis , cutting of DNA by restriction endonucleases results in fragments of DNA , DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode , under an electric field through agarose , DNA fragments separate (resolve) according to their size through sieving effect (provided by the agarose gel.) 

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