Question

What was the challenge for production of insulin using rDNA techniques ? How did Eli Lilly produce insulin using rDNA technology ?

Answer 1

The challenge for production of insulin using r DNA technique was getting insulin assembled into a mature form 
• Prepared two DNA sequence corresponding to A and B chains of human insulin.
• introduced them in plasmids of E.coli to produce insulin chains.
• chains A and B were produced separately.
• extracted and combined by creating disulfide bonds to form human insulin