Question

Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

Answer 1

i. Restriction endonucleases: for cutting the desired DNA at desired places
ii. Gel electrophoresis: for separating the desired DNA fragments
iii. Ligase enzyme: for creating recombinant DNA molecule.
iv. DNA delivery system: like electroporation, microinjection, gene gun method.
v. Competant host (usually bacteria / yeast): to take up recombinant DNA.